Research
We investigate the molecular mechanisms that regulate pain perception, with a particular emphasis on the neuronal membrane proteasome (NMP) in sensory neuron function.
Investigating the molecular mechanisms underlying sensory neuron excitability and signaling.
Dorsal root ganglion (DRG) and trigeminal ganglion (TG) neurons detect and transmit information about touch, temperature, itch, and pain. Our laboratory investigates the molecular mechanisms that regulate sensory neuron function using primary neuron cultures, calcium imaging, and behavioral approaches.
Understanding how the NMP modulates sensory pathway-dependent sensitivity.
The neuronal membrane proteasome (NMP) is a membrane-associated proteolytic complex found in subsets of peripheral sensory neurons. We investigate how NMP activity regulates sensory neuron signaling and sensitivity to different sensory stimuli.
Understanding how neuropathy alters NMP function and how these changes contribute to neuropathic hypersensitivity and pain.
Peripheral nerve injury can alter sensory neuron signaling and produce persistent hypersensitivity. We investigate how neuropathy changes NMP abundance and function, and whether NMP-dependent signaling contributes to altered sensory responses and neuropathic pain.
Methods
We combine techniques across scales — from molecular signaling to whole-animal behaviour.
Gene knockdown and overexpression, protein biochemistry, mass spectrometry, and single-cell RNA sequencing.
Real-time visualisation of neuronal activity using fluorescent calcium indicators.
Primary cultures of dorsal root ganglion (DRG) and trigeminal ganglion (TG) neurons to investigate sensory neuron function and signaling.
Mouse models to investigate normal sensation and neuropathy-associated alterations in pain, touch, and itch.
Mass spectrometry to identify proteasome substrates and protein interactions.